Agrobacterium mediated gene transformation in plants
For more information, log on to- http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html Transfer of T-DNA into the plant cell A: Agrobacterium tumefaciens B: Agrobacterium genome C: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genes D: Plant cell E: Mitochondria F: Chloroplast G: Nucleus The T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS). In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognised by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. VIP1 also appears to be an important protein in the process, possibly acting as an adapter to bring the VirE2 to the importin. Once inside the nucleus, VIP2 may target the T-DNA to areas of chromatin that are being actively transcribed, so that the T-DNA can integrate into the host genome. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia. Link- http://en.wikipedia.org/wiki/Main_Page
Comments
-
Which chapter is the ppt used in this video is present on?
P.S. Excellent explanations -
So the VirF-operon codes for "signal peptides" which will allow the T-complex to enter the Nuclear Envelope of the plant cell?
-
Don't wanna be mean, but you need to work on your speaking skills. The thick accent is at times hard to understand, and this issue is amplified by your wet pronunciation. The accent is not easily dealt with, but I believe the wet pronunciation can be minimised. You also weren't very clear at some moments when you kinda "ran off" with supplementary remarks, going faster as well as quieter.
Working on these points would certainly improve the quality of your presentations and the ease of learning from them. :) -
all videos are very nice for m.sc. students. excellent presentation.
-
Fuck this, where's part 1? Why does the video start in some random slide? Seriously dude, what the fuck, you put a lot of work into this, can't you go an extra mile and take a minute to edit properly?
-
Before transfection the T DNA gets converted into ss DNA , is this DNA is cDNA ???????
-
how Agro. differentiate between transformed cell and untransformed one ?
-
nice presentation thanks for do this...:-)
-
Agro CAN indeed be used in cereal crop transformation.
-
yes. you are right.
-
how do the agrobacterium differentiate between transfected and non-affected cells? Is it through the presence/absence of nopaline/octopine?
19m 1sLenght
44Rating